کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2049447 | 1074128 | 2007 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of nuclear localization and nuclear export signals of yeast actin-binding protein Pan1
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Characterization of nuclear localization and nuclear export signals of yeast actin-binding protein Pan1 Characterization of nuclear localization and nuclear export signals of yeast actin-binding protein Pan1](/preview/png/2049447.png)
چکیده انگلیسی
Pan1 is an actin patch-associated protein involved in endocytosis. Our studies revealed that in oleate-grown cells Pan1 is located in the nucleus as well as in patches. One of three putative nuclear localization signals (NLS) of Pan1, NLS2, directed β-galactosidase (β-gal) to the nucleus. However, GFP-Pan1886−1219, containing NLS2, was found in the cytoplasm indicating that it may contain a nuclear export signal (NES). A putative Pan1 NES, overlapping with NLS3, re-addressed NLSH2B-NES/NLS3-β-gal from the nucleus to the cytoplasm. Inactivation of the NES allowed NLS3 to be effective. Thus, Pan1 contains functional NLSs and a NES and appears to shuttle in certain circumstances.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 581, Issue 28, 27 November 2007, Pages 5371–5376
Journal: FEBS Letters - Volume 581, Issue 28, 27 November 2007, Pages 5371–5376
نویسندگان
Joanna Kamińska, Magdalena Sędek, Monika Wysocka-Kapcińska, Teresa Żołądek,