کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2049456 | 1074128 | 2007 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Locked 5Zs-biliverdin blocks the Meta-RA to Meta-RC transition in the functional cycle of bacteriophytochrome Agp1
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The bacteriophytochrome Agp1 was reconstituted with a locked 5Zs-biliverdin in which the C4C5 and C5–C6 bonds of the methine bridge between rings A and B are fixed in the Z and syn configuration/conformation, respectively. In Agp1-5Zs the photoconversion proceeds via the Lumi-R intermediate to Meta-RA, but the following millisecond-transition to Meta-RC is blocked. Consistently, no transient proton release was detected. The photoconversion of Agp1-5Zs is apparently arrested in a Meta-RA-like intermediate, since the subsequent syn to anti rotation around the C5–C6 bond is prevented by the lock. The Meta-RA-like photoproduct was characterized by its distinctive CD spectrum suggesting a reorientation of ring D.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 581, Issue 28, 27 November 2007, Pages 5425–5429
Journal: FEBS Letters - Volume 581, Issue 28, 27 November 2007, Pages 5425–5429
نویسندگان
Sven Seibeck, Berthold Borucki, Harald Otto, Katsuhiko Inomata, Htoi Khawn, Hideki Kinoshita, Norbert Michael, Tilman Lamparter, Maarten P. Heyn,