کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2049464 1074128 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Histological mapping of biochemical changes in solid tumors by FT-IR spectral imaging
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Histological mapping of biochemical changes in solid tumors by FT-IR spectral imaging
چکیده انگلیسی

Fourier-transform infrared (FT-IR) spectral imaging was used for analyzing biochemical changes in tumor cells. Metabolic parameters of human lung A549/8 adenocarcinoma and U87 glioma cells were compared under stress conditions in culture along with tumor progression after cell implantation onto the chick embryo chorio-allantoic membrane. In cell culture, glucose consumption and lactic acid release were higher in U87 cells. A549/8 cells were less sensitive to oxidative stress as observed through changes in fatty acyl chains. In vivo biochemical mapping of highly (U87) vs. poorly (A549/8) angiogenic tumors provided results comparable to culture models. Therefore, FT-IR imaging allows detecting subtle chemical changes in tumors, which might be useful for diagnosis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 581, Issue 28, 27 November 2007, Pages 5469–5474
نویسندگان
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