کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2049466 | 1074128 | 2007 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Creation of intercellular bonds by anchoring protein ligands to membranes using the diphtheria toxin T domain
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Creation of intercellular bonds by anchoring protein ligands to membranes using the diphtheria toxin T domain Creation of intercellular bonds by anchoring protein ligands to membranes using the diphtheria toxin T domain](/preview/png/2049466.png)
چکیده انگلیسی
We describe the creation of cell adhesion mediated by cell surface engineering. The Flt3-ligand was fused to a membrane anchor made of the diphtheria toxin translocation domain. The fusion protein was attached to the surface of a cell by an acid pulse. Contact with another cell expressing the receptor Flt3 lead to its activation. This activity involved direct cell–cell contact. A mean force of 20 nN was needed to separate functionalized cells after 5 min of contact. Overall, we showed that it is possible to promote specific cell–cell adhesion by attaching protein ligands at the surface of cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 581, Issue 28, 27 November 2007, Pages 5480–5484
Journal: FEBS Letters - Volume 581, Issue 28, 27 November 2007, Pages 5480–5484
نویسندگان
Aurélie Perier, Christine Gourier, Sylvain Pichard, Julien Husson, Evelyne Lajeunesse, Aurélie Babon, André Menez, Daniel Gillet,