کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2049768 1074142 2009 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Ab initio protein modelling reveals novel human MIT domains
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Ab initio protein modelling reveals novel human MIT domains
چکیده انگلیسی

Database searches can fail to detect all truly homologous sequences, particularly when dealing with short, highly sequence diverse protein families. Here, using microtubule interacting and transport (MIT) domains as an example, we have applied an approach of profile–profile matching followed by ab initio structure modelling to the detection of true homologues in the borderline significant zone of database searches. Novel MIT domains were confidently identified in USP54, containing an apparently inactive ubiquitin carboxyl-terminal hydrolase domain, a katanin-like ATPase KATNAL1, and an uncharacterized protein containing a VPS9 domain. As a proof of principle, we have confirmed the novel MIT annotation for USP54 by in vitro profiling of binding to CHMP proteins.Structured summaryUSP8 binds:CHMPs 1A 1B 2A 2B 4CUSP54 binds:CHMPs 1B 2A 2B 4C 6

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 583, Issue 5, 4 March 2009, Pages 872–878
نویسندگان
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