کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2049787 1074143 2009 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Real time analysis of lipoprotein transfer from LolA to LolB by means of surface plasmon resonance
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Real time analysis of lipoprotein transfer from LolA to LolB by means of surface plasmon resonance
چکیده انگلیسی

Lipoproteins of Escherichia coli are sorted to the outer membrane through a pathway composed of five Lol proteins. LolA transports lipoproteins released from the inner membrane by LolCDE to LolB on the outer membrane via the periplasm. Interaction between LolA and LolB was speculated to be strong when LolA binds lipoprotein. However, due to a lack of a sensitive method, the kinetics of this reaction have not been examined in detail. We report here the detection of lipoprotein transfer in real time by means of surface plasmon resonance. The kinetic parameters of lipoprotein transfer were determined with wild-type LolA and a mutant defective in it.Structured summaryMINT-7259948: mlolB (uniprotkb:P61320) binds (MI:0407) to pal (uniprotkb:P0A912) by surface plasmon resonance (MI:0107)

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 583, Issue 18, 17 September 2009, Pages 2987–2990
نویسندگان
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