کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2049791 | 1074143 | 2009 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: PANDER binds to the liver cell membrane and inhibits insulin signaling in HepG2 cells PANDER binds to the liver cell membrane and inhibits insulin signaling in HepG2 cells](/preview/png/2049791.png)
PANDER is a cytokine co-secreted with insulin from islet β-cells. To date, the physiological function of PANDER remains largely unknown. Here we show that PANDER binds to the liver membrane by 125I-PANDER saturation and competitive binding assays. In HepG2 cells, pre-treatment with PANDER ranging from 4 pM to 4 nM for 8 h resulted in a maximal inhibition of insulin-stimulated activation of insulin receptor and insulin receptor substrate 1 by 52% and 63%, respectively. Moreover, PANDER treatment also reduced insulin-stimulated PI3K and pAkt levels by 55% and 48%, respectively. In summary, we have identified the liver as a novel target for PANDER, and PANDER may be involved in the progression of diabetes by regulating hepatic insulin signaling pathways.
Journal: FEBS Letters - Volume 583, Issue 18, 17 September 2009, Pages 3009–3015