کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2050008 1074150 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Spatial and temporal down-regulation of transgene expression using the TRSID-silencer in mice: Application to Prnp
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Spatial and temporal down-regulation of transgene expression using the TRSID-silencer in mice: Application to Prnp
چکیده انگلیسی

Spatial and temporal control of ovine prion protein (Prnp) gene expression was achieved in mice using two transgenes: a Prnp minigene with tet-operator sequences inserted 5′ to exon 1 and a mouse neurofilament genomic clone carrying the chimeric-repressor TRSID cDNA. In bi-transgenic mice, ovine PrPC expression could be reversibly controlled in neuronal cells by doxycycline treatment whereas it remains constant in other cell types. Overall, this model opens opportunities to assess the involvement of cell types in prion diseases and PrP physiological function. It demonstrates the potentiality of the TRSID-silencer to precisely control temporal and spatial gene expression in vivo.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 582, Issue 15, 25 June 2008, Pages 2219–2224
نویسندگان
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