کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2050237 1074162 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Extracellular catalase activity protects cysteine cathepsins from inactivation by hydrogen peroxide
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Extracellular catalase activity protects cysteine cathepsins from inactivation by hydrogen peroxide
چکیده انگلیسی

The resistance of secreted cysteine cathepsins to peroxide inactivation was evaluated using as model THP-1 cells. Differentiated cells released mostly cathepsin B, but also cathepsins H, K, and L, with a maximum of endopeptidase activity at day 6. Addition of non-cytotoxic concentrations of H2O2 did not affect mRNA expression levels and activity of cathepsins, while the catalase activity remained also unchanged, consistently with RT-PCR analysis. Conversely inhibition of extracellular catalase led to a striking inactivation of secreted cysteine cathepsins by H2O2. This report suggests that catalase may participate in the protection of extracellular cysteine proteases against peroxidation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 582, Issue 9, 16 April 2008, Pages 1307–1312
نویسندگان
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