کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2050241 1074162 2008 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Promoted evolution of a shortened variant of heme A synthase in the membrane of Bacillus subtilis
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Promoted evolution of a shortened variant of heme A synthase in the membrane of Bacillus subtilis
چکیده انگلیسی

Bacillus subtilis heme A synthase is a membrane protein with 8 transmembrane segments. By using a two-step mutagenesis approach we have generated and selected a fully functional enzyme protein variant with a seven residue internal deletion. The biochemical properties of the shortened variant are similar to those of the normal enzyme. This could indicate that residue H209 in the mutant protein substitutes for the missing H216 as an axial ligand to the heme iron. Our results provide insight in routes of membrane protein evolution and the structure of heme A synthases.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 582, Issue 9, 16 April 2008, Pages 1330–1334
نویسندگان
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