کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2050305 1074164 2006 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Phosphoinositide synthesis and degradation in isolated rat liver peroxisomes
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Phosphoinositide synthesis and degradation in isolated rat liver peroxisomes
چکیده انگلیسی

Analyzing peroxisomal phosphoinositide (PId#) synthesis in highly purified rat liver peroxisomes we found synthesis of phosphatidylinositol 4-phosphate (PtdIns4P), PtdIns(4,5)P2 and PtdIns(3,5)P2. PtdIns3P was hardly detected in vitro, however, was observed in vivo after [32P]-phosphate labeling of primary rat hepatocytes. In comparison with other subcellular organelles peroxisomes revealed a unique PId pattern suggesting peroxisomal specificity of the observed synthesis. Use of phosphatase inhibitors enhanced the amount of PtdIns4P. The results obtained provide evidence that isolated rat liver peroxisomes synthesize PIds and suggest the association of PId 4-kinase and PId 5-kinase and PId 4-phosphatase activities with the peroxisomal membrane.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 580, Issue 25, 30 October 2006, Pages 5917–5924
نویسندگان
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