کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2050383 | 1074168 | 2009 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The MerE protein encoded by transposon Tn21 is a broad mercury transporter in Escherichia coli
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
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![عکس صفحه اول مقاله: The MerE protein encoded by transposon Tn21 is a broad mercury transporter in Escherichia coli The MerE protein encoded by transposon Tn21 is a broad mercury transporter in Escherichia coli](/preview/png/2050383.png)
چکیده انگلیسی
In order to clarify the physiological role of the merE gene of transposon Tn21, a pE4 plasmid that contained the merR gene of plasmid pMR26 from Pseudomonas strain K-62, and the merE gene of Tn21 from the Shigella flexneri plasmid NR1 (R100) was constructed. Bacteria with plasmid pE4 (merR-o/p-merE) were more hypersensitive to CH3Hg(I) and Hg(II), and took up significantly more CH3Hg(I) and Hg(II), than the isogenic strain. The MerE protein encoded by pE4 was localized in the membrane cell fraction, but not in the soluble fraction. Based on these experimental results, we suggest for the first time that the merE gene is a broad mercury transporter mediating the transport of both CH3Hg(I) and Hg(II) across the bacterial membrane.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 583, Issue 7, 2 April 2009, Pages 1127–1131
Journal: FEBS Letters - Volume 583, Issue 7, 2 April 2009, Pages 1127–1131
نویسندگان
Masako Kiyono, Yuka Sone, Ryosuke Nakamura, Hidemitsu Pan-Hou, Kou Sakabe,