کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2050387 1074168 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The ADP-ribosylation of Sulfolobus solfataricus Sso7 modulates protein/DNA interactions in vitro
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
The ADP-ribosylation of Sulfolobus solfataricus Sso7 modulates protein/DNA interactions in vitro
چکیده انگلیسی

The 7 kDa Sso7 is a basic protein particularly abundant in Sulfolobus solfataricus and is involved in DNA assembly. This protein undergoes in vitro ADP-ribosylation by an endogenous poly(ADP-ribose) polymerase-like enzyme. The circular dichroism spectrum of purified ADP-ribosylated Sso7 shows that this modification stabilizes the prevalent protein β-conformation, as suggested by shifting of negative ellipticity minimum to 220 nm. Moreover, a short ADP-ribose chain (up to 6-mers) bound to Sso7 is able to reduce drastically the thermoprotective and DNA condensing ability of the protein, suggesting a possible regulatory role of ADP-ribosylation in sulfolobal DNA organization.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 583, Issue 7, 2 April 2009, Pages 1154–1158
نویسندگان
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