کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2050503 1074172 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Crystal structure of a family I.3 lipase from Pseudomonas sp. MIS38 in a closed conformation
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Crystal structure of a family I.3 lipase from Pseudomonas sp. MIS38 in a closed conformation
چکیده انگلیسی

The crystal structure of a family I.3 lipase from Pseudomonas sp. MIS38 in a closed conformation was determined at 1.5 Å resolution. This structure highly resembles that of Serratia marcescens LipA in an open conformation, except for the structures of two lids. Lid1 is anchored by a Ca2+ ion (Ca1) in an open conformation, but lacks this Ca1 site and greatly changes its structure and position in a closed conformation. Lid2 forms a helical hairpin in an open conformation, but does not form it and covers the active site in a closed conformation. Based on these results, we discuss on the lid-opening mechanism.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 581, Issue 26, 30 October 2007, Pages 5060–5064
نویسندگان
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