کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2050537 | 1074173 | 2009 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Functional characterization of the DnaK chaperone system from the archaeon Methanothermobacter thermautotrophicusΔH
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Functional characterization of the DnaK chaperone system from the archaeon Methanothermobacter thermautotrophicusΔH Functional characterization of the DnaK chaperone system from the archaeon Methanothermobacter thermautotrophicusΔH](/preview/png/2050537.png)
چکیده انگلیسی
We characterized the biochemical and functional properties of the DnaK system from the archaeon Methanothermobacter thermautotrophicus ΔH. In contrast to the eubacterial chaperone components the archaeal Hsp70 system shows thermal transitions only slightly above the optimal environmental temperature (65 °C). Nevertheless, it prevents aggregation of luciferase in the physiological temperature range of the organism, but is also fully functional at 30 °C in luciferase refolding. Additionally, GrpEM.th. and DnaJM.th. substitute their eubacterial counterparts whereas DnaKM.th. is only functional with its native cochaperones which could be attributed to a functional specialization of the eubacterial chaperones during evolution.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 583, Issue 3, 4 February 2009, Pages 573–578
Journal: FEBS Letters - Volume 583, Issue 3, 4 February 2009, Pages 573–578
نویسندگان
Simone L. Popp, Jochen Reinstein,