کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2050729 | 1074180 | 2007 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: ABCA3-mediated choline-phospholipids uptake into intracellular vesicles in A549 cells ABCA3-mediated choline-phospholipids uptake into intracellular vesicles in A549 cells](/preview/png/2050729.png)
ABCA3 is proposed to function as a lung surfactant lipid transporter. Here we report ABCA3-dependent lipid uptake into intracellular vesicles in lung adenocarcinoma A549 cells. A549 cells stably expressing GFP-tagged wild-type ABCA3 (A549/ABCA3WT) had larger LAMP3-positive vesicles than their parental cells as well as A549 cells expressing a Walker A motif mutant (A549/ABCA3N568D). The choline-phospholipids level in A549/ABCA3WT was increased 1.25-fold compared to that in A549 and A549/ABCA3N568D cells, while the cholesterol levels were similar. Sucrose gradient fractionation analysis in A549/ABCA3WT cells revealed that choline-phospholipids were enriched in low-density and nile red-positive vesicles. Electronmicroscopic analysis showed multilamellar vesicles in A549/ABCA3WT cells. These results indicate that ABCA3 mediates ATP-dependent choline-phospholipids uptake into intracellular vesicles.
Journal: FEBS Letters - Volume 581, Issue 17, 10 July 2007, Pages 3139–3144