کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2050948 1074186 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Site-directed mutagenesis studies of acetylglutamate synthase delineate the site for the arginine inhibitor
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Site-directed mutagenesis studies of acetylglutamate synthase delineate the site for the arginine inhibitor
چکیده انگلیسی

N-acetyl-l-glutamate synthase (NAGS), the first enzyme of bacterial/plant arginine biosynthesis and an essential activator of the urea cycle in animals, is, respectively, arginine-inhibited and activated. Site-directed mutagenesis of recombinant Pseudomonas aeruginosa NAGS (PaNAGS) delineates the arginine site in the PaNAGS acetylglutamate kinase-like domain, and, by extension, in human NAGS. Key residues for glutamate binding are identified in the acetyltransferase domain. However, the acetylglutamate kinase-like domain may modulate glutamate binding, since one mutation affecting this domain increases the Km for glutamate. The effects on PaNAGS of two mutations found in human NAGS deficiency support the similarity of bacterial and human NAGSs despite their low sequence identity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 582, Issue 7, 2 April 2008, Pages 1081–1086
نویسندگان
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