کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2050957 | 1074186 | 2008 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Peroxynitrite inactivates human-tissue inhibitor of metalloproteinase-4 Peroxynitrite inactivates human-tissue inhibitor of metalloproteinase-4](/preview/png/2050957.png)
Peroxynitrite, via post-translational modifications to target proteins, contributes to cardiovascular injury and cancer. Since tissue inhibitor of metalloproteinase-4 (TIMP-4), the activity of which is impaired in both pathological conditions, has several amino acid residues susceptible to peroxynitrite, we investigated its role as a potential target of peroxynitrite. Peroxynitrite-induced nitration and oligomerization of TIMP-4 attenuated its inhibitory activity against MMP-2 activity and endothelial or tumor cell invasiveness. Moreover, cell treatment with peroxynitrite promoted the nitration of endogenous TIMP-4. HPLC/ESI-MS/MS analysis of peroxynitrite-treated TIMP-4 showed modifications at Y114, Y195, Y188 and Y190. In conclusion, TIMP-4 nitration might be a potential mechanism contributing to cardiovascular disease and cancer.
Journal: FEBS Letters - Volume 582, Issue 7, 2 April 2008, Pages 1135–1140