کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2051051 1074189 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization of homogentisate prenyltransferases involved in plastoquinone-9 and tocochromanol biosynthesis
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Characterization of homogentisate prenyltransferases involved in plastoquinone-9 and tocochromanol biosynthesis
چکیده انگلیسی

A cDNA of Chlamydomonas reinhardtii encoding a plastidial homogentisate prenyltransferase was identified. Functional expression studies in Escherichia coli revealed that the enzyme possessed properties similar to the prenyltransferase of Arabidopsis thaliana encoded by At3g11950 but different from the phytyltransferases of A. thaliana and Synechocystis. Unlike the phytyltransferases, the C. reinhardtii and the respective A. thaliana enzyme showed highest activities with solanesyl diphosphate, but were hardly active with phytyl diphosphate. Hence, these data provide evidence that the latter represent homogentisate solanesyltransferases involved in plastoquinone-9 biosynthesis. Overexpression of At3g11950 in A. thaliana, however, suggests that the solanesyltransferase can affect tocopherol biosynthesis as well.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 580, Issue 22, 2 October 2006, Pages 5357–5362
نویسندگان
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