کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2051242 1074195 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Copper complexes of (−)-epicatechin gallate and (−)-epigallocatechin gallate act as inhibitors of Ribonuclease A
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Copper complexes of (−)-epicatechin gallate and (−)-epigallocatechin gallate act as inhibitors of Ribonuclease A
چکیده انگلیسی

Green tea polyphenols, which have the ability to inhibit angiogenesis, form complexes with Cu(II), a known potent stimulator of blood vessel proliferation. Copper complexes of (−)-epicatechin gallate and (−)-epigallocatechin gallate were found to inhibit the enzymatic activity of Ribonuclease A (RNase A) as revealed by an agarose gel based assay and urea denatured gel electrophoresis. The copper complexes were found to be non-competitive inhibitors of RNase A with inhibition constants in the micromolar range. Changes in the secondary structure of the protein are found to occur due to the interaction as revealed from Fourier transform infrared and circular dichroism studies.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 580, Issue 19, 21 August 2006, Pages 4703–4708
نویسندگان
, , , ,