p14ARF interacts with N-Myc and inhibits its transcriptional activity

In this study, we report that the human p14ARF associates in vivo with the N-Myc and inhibits N-Myc mediated transcriptional activation. We have determined that the region (aa 140–300) encompassing the N-Myc BoxIII is required for efficient interaction in vivo. Furthermore, we demonstrate that in the SK-N-BE neuroblastoma cell line p14ARF over-expression delocalized N-Myc from the nucleoplasm into nucleoli and that N-Myc regions required for interaction with p14ARF are also important for nucleoli co-localization. Finally, we determine that the N-terminal region of the p14ARF protein is involved in binding to c-Myc and N-Myc proteins.