کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2051560 1074203 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Fabrication of a dual substrate display to test roles of cell adhesion proteins in vesicle targeting to plasma membrane domains
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Fabrication of a dual substrate display to test roles of cell adhesion proteins in vesicle targeting to plasma membrane domains
چکیده انگلیسی
While much is known of the molecular machinery involved in protein sorting during exocytosis, less is known about the spatial regulation of exocytosis at the plasma membrane (PM). This study outlines a novel method, dual substrate display, used to formally test the hypothesis that E-cadherin-mediated adhesion directs basolateral vesicle exocytosis to specific sites at the PM. We show that vesicles containing the basolateral marker protein VSV-G preferentially target to sites of adhesion to E-cadherin rather than collagen VI or a control peptide. These results support the hypothesis that E-cadherin adhesion initiates signaling at the PM resulting in targeted sites for exocytosis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 581, Issue 23, 18 September 2007, Pages 4539-4543
نویسندگان
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