کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2051628 1074206 2006 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Arabidopsis GIGANTEA protein is post-transcriptionally regulated by light and dark
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Arabidopsis GIGANTEA protein is post-transcriptionally regulated by light and dark
چکیده انگلیسی

GIGANTEA (GI) is a key regulator of photoperiodic flowering in Arabidopsis and encodes a protein with no domains of known biochemical function. Expression of GI mRNA is controlled by the circadian clock, but GI protein accumulation has not been previously investigated. We generated plants that produced functional epitope-tagged GI to enable us to track the protein through the daily cycle. Here we show that GI protein levels oscillate when either constitutively overexpressed or driven by its promoter and that its accumulation is modulated by day length as well as by phase-specific factors. Also, we demonstrate that one of the mechanisms underlying GI protein oscillation occurs post-translationally via dark-induced proteolysis by the 26S proteasome.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 580, Issue 5, 20 February 2006, Pages 1193–1197
نویسندگان
, , , ,