کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2051647 1074206 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Microtubule binding proteins CLIP-170, EB1, and p150Glued form distinct plus-end complexes
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Microtubule binding proteins CLIP-170, EB1, and p150Glued form distinct plus-end complexes
چکیده انگلیسی

Microtubule plus-end proteins CLIP-170 and EB1 dynamically track the tips of growing microtubules in vivo. Here we examine the association of these proteins with microtubules in vitro. CLIP-170 binds tubulin dimers and co-assembles into growing microtubules. EB1 binds tubulin dimers more weakly, so no co-assembly is observed. However, EB1 binds to CLIP-170, and forms a co-complex with CLIP-170 and tubulin that is recruited to growing microtubule plus ends. The interaction between CLIP-170 and EB1 is competitively inhibited by the related CAP-Gly protein p150Glued, which also localizes to microtubule plus ends in vivo. Based on these observations, we propose a model in which the formation of distinct plus-end complexes may differentially affect microtubule dynamics in vivo.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 580, Issue 5, 20 February 2006, Pages 1327–1332
نویسندگان
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