کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2051763 1074209 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Possible direct involvement of the active-site [4Fe–4S] cluster of the GcpE enzyme from Thermus thermophilus in the conversion of MEcPP
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Possible direct involvement of the active-site [4Fe–4S] cluster of the GcpE enzyme from Thermus thermophilus in the conversion of MEcPP
چکیده انگلیسی

The GcpE enzyme converts 2-C-methyl-d-erythritol-2,4-cyclodiphosphate (MEcPP) into (E)-4-hydroxy-3-methyl-but-2-enyl diphosphate (HMBPP) in the penultimate step of the DOXP pathway for isoprene biosynthesis. Purification of the enzyme under exclusion of air leads to a preparation that contains solely [4Fe–4S] clusters. Kinetic studies showed that in the presence of the artificial reductant dithionite and MEcPP a new transient iron–sulfur-based signal is detected in electron paramagnetic resonance (EPR) spectroscopy. Similarity of this EPR signal to that detected in ferredoxin:thioredoxin reductase indicates that during the reaction an intermediate is directly bound to the active-site cluster.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 581, Issue 2, 23 January 2007, Pages 279–283
نویسندگان
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