کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2052010 1074218 2007 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Active Gαq subunits and M3 acetylcholine receptors promote distinct modes of association of RGS2 with the plasma membrane
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Active Gαq subunits and M3 acetylcholine receptors promote distinct modes of association of RGS2 with the plasma membrane
چکیده انگلیسی

RGS proteins accelerate the GTPase activity of heterotrimeric G proteins at the plasma membrane. Association of RGS proteins with the plasma membrane can be mediated by interactions with other membrane proteins and by direct interactions with the lipid bilayer. Here we use fluorescence recovery after photobleaching (FRAP) to characterize interactions between RGS2 and M3 acetylcholine receptors (M3Rs), Gα subunits and the lipid bilayer. Active Gαq and M3Rs both recruited RGS2-EGFP to the plasma membrane. RGS2-EGFP remained bound to the plasma membrane between interactions with active Gαq, but rapidly exchanged between membrane-associated and cytosolic pools when recruited by M3Rs.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 581, Issue 4, 20 February 2007, Pages 764–770
نویسندگان
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