کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2052188 1543466 2006 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
ATP binding site in the plant ADP-glucose pyrophosphorylase large subunit
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
ATP binding site in the plant ADP-glucose pyrophosphorylase large subunit
چکیده انگلیسی

The ATP binding region in the catalytically inactive large subunit (LS) of the potato tuber ADP-glucose pyrophosphorylase was identified and investigated. Mutations at the ATP binding significantly affected not only the apparent affinities for ATP and Glc-1-P, and catalytic rate but also in many instances, sensitivity to 3-phosphoglycerate. The catalytic rates of the LS mutant enzymes correlated most strongly with changes in the affinity toward ATP, a relationship substantiated by photoaffinity labeling studies with azido-ATP analog. These results indicate that the LS, although catalytically defective, interacts cooperatively with the catalytic small subunit in binding substrates and effectors and, in turn, influencing net catalysis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 580, Issues 28–29, 11 December 2006, Pages 6741–6748
نویسندگان
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