کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2052409 | 1074230 | 2005 | 7 صفحه PDF | دانلود رایگان |
Bacillus thuringiensis subs israelensis produces Cry toxins active against mosquitoes. Receptor binding is a key determinant for specificity of Cry toxins composed of three domains. We found that exposed loop α-8 of Cry11Aa toxin, located in domain II, is an important epitope involved in receptor interaction. Synthetic peptides corresponding to exposed regions in domain II (loop α-8, β-4 and loop 3) competed binding of Cry11Aa to membrane vesicles from Aedes aegypti midgut microvilli. The role of loop α-8 of Cry11A in receptor interaction was demonstrated by phage display and site-directed mutagenesis. We isolated a peptide-displaying phage (P5.tox), that recognizes loop α-8 in Cry11Aa, interferes interaction with the midgut receptor and attenuates toxicity in bioassay. Loop α-8 mutants affected in toxicity and receptor binding were characterized.
Journal: FEBS Letters - Volume 579, Issue 17, 4 July 2005, Pages 3508–3514