کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2052456 | 1074230 | 2005 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Nitrite as the major source of nitric oxide production by Arabidopsis thaliana in response to Pseudomonas syringae Nitrite as the major source of nitric oxide production by Arabidopsis thaliana in response to Pseudomonas syringae](/preview/png/2052456.png)
The origin of nitric oxide (NO) in plants is unclear and an NO synthase (NOS)-like enzyme and nitrate reductase (NR) are claimed as potential sources. Here we used wild-type and NR-defective double mutant plants to investigate NO production in Arabidopsis thaliana in response to Pseudomonas syringae pv maculicola. NOS activity increased substantially in leaves inoculated with P. syringae. However, electron paramagnetic resonance experiments showed a much higher NO formation that was dependent on nitrite and mitochondrial electron transport rather than on arginine or nitrate. Overall, these results indicate that NOS, NR and a mitochondrial-dependent nitrite-reducing activity cooperate to produce NO during A. thaliana–P. syringae interaction.
Journal: FEBS Letters - Volume 579, Issue 17, 4 July 2005, Pages 3814–3820