کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2052462 1074230 2005 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Transmembrane homodimerization of receptor-like protein tyrosine phosphatases
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Transmembrane homodimerization of receptor-like protein tyrosine phosphatases
چکیده انگلیسی

Receptor-like protein tyrosine phosphatases (RPTPs) are type I integral membrane proteins. Together with protein tyrosine kinases, RPTPs regulate the phosphotyrosine levels in the cell. Studies of two RPTPs, CD45 and PTPα, have provided strong evidence that dimerization leads to inactivation of the receptors, and that the dimerization of PTPα involves interactions in the transmembrane domain (TMD). Using the TOXCAT assay, a genetic approach for analyzing TM interactions in Escherichia coli membranes, we show that the TMD of RPTPs interact in the membrane, albeit to different extents. Using fusion proteins of TMDs, we also observe an equilibrium between monomer and dimer in sodium dodecyl sulfate (SDS) micelles. Through a mutational study of the DEP1 TMD, we demonstrate that these interactions are specific. Taken together, our results define a subset of the RPTP family in which TM homodimerization may act as a mediator of protein function.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 579, Issue 17, 4 July 2005, Pages 3855–3858
نویسندگان
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