کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2052603 | 1074234 | 2006 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The transition metal chelator nicotianamine is synthesized by filamentous fungi
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: The transition metal chelator nicotianamine is synthesized by filamentous fungi The transition metal chelator nicotianamine is synthesized by filamentous fungi](/preview/png/2052603.png)
چکیده انگلیسی
Nicotianamine is an important metal ligand in plants. Surprisingly, recent genome sequencing revealed that ascomycetes encode proteins with similarity to plant nicotianamine synthases (NAS). By expression in a Zn2+-hypersensitive fission yeast mutant we show for a protein from Neurospora crassa that it indeed possesses NAS activity. Using electrospray-ionization-quadrupole-time-of-flight mass spectrometry we prove the formation of nicotianamine in N. crassa. Transcript level is strongly upregulated under Zn deficiency as shown by real-time PCR. These findings demonstrate that nicotianamine is more widespread in nature than anticipated and provide further evidence for a function of nicotianamine as a cytosolic chelator of Zn2+ ions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 580, Issue 13, 29 May 2006, Pages 3173–3178
Journal: FEBS Letters - Volume 580, Issue 13, 29 May 2006, Pages 3173–3178
نویسندگان
Aleksandra Trampczynska, Christoph Böttcher, Stephan Clemens,