کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2052717 1074238 2005 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of Escherichia coli K12 YdcW protein as a γ-aminobutyraldehyde dehydrogenase
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Identification of Escherichia coli K12 YdcW protein as a γ-aminobutyraldehyde dehydrogenase
چکیده انگلیسی

γ-Aminobutyraldehyde dehydrogenase (ABALDH) from wild-type E. coli K12 was purified to apparent homogeneity and identified as YdcW by MS-analysis. YdcW exists as a tetramer of 202 ± 29 kDa in the native state, a molecular mass of one subunit was determined as 51 ± 3 kDa. Km parameters of YdcW for γ-aminobutyraldehyde, NAD+ and NADP+ were 41 ± 7, 54 ± 10 and 484 ± 72 μM, respectively. YdcW is the unique ABALDH in E. coli K12. A coupling action of E. coli YgjG putrescine transaminase and YdcW dehydrogenase in vitro resulted in conversion of putrescine into γ-aminobutyric acid.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 579, Issue 19, 1 August 2005, Pages 4107–4112
نویسندگان
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