کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2052758 | 1074239 | 2006 | 7 صفحه PDF | دانلود رایگان |
We developed a new generic fluorescence sensing technology based on the change of relative intensities between two well-separated emission bands of the novel functional 3-hydroxychromone (3HC) dyes. A greatly enhanced self-calibrating wavelength-ratiometric response is obtained to all major types of non-covalent interactions that can be used in sensing – to polarity, hydrogen bonding ability and to local electrostatic fields. This technology may find a broad range of applications – from homogeneous assays in solutions to microarrays, microfluidic devices, nanosensors and whole cell imaging systems. It allows transforming micelles or phospholipid vesicles into nanosensor devices. In cellular research a high sensitivity to membrane potentials can be obtained and the membrane changes during apoptosis detected.
Journal: FEBS Letters - Volume 580, Issue 12, 22 May 2006, Pages 2951–2957