کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2052892 1074244 2006 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Substrate-induced double sided H-bond network as a means of domain closure in 3-phosphoglycerate kinase
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Substrate-induced double sided H-bond network as a means of domain closure in 3-phosphoglycerate kinase
چکیده انگلیسی

Closure of the two domains of 3-phosphoglycerate kinase, upon substrate binding, is essential for the enzyme function. The available crystal structures cannot provide sufficient information about the mechanism of substrate assisted domain closure and about the requirement of only one or both substrates, since lattice forces may hinder the large scale domain movements. In this study the known X-ray data, obtained for the open and closed conformations, were probed by solution small-angle X-ray scattering experiments. The results prove that binding of both substrates is essential for domain closure. Molecular graphical analysis, indeed, reveals formation of a double-sided H-bond network, which affects substantially the shape of the main molecular hinge at β-strand L, under the concerted action of both substrates.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 580, Issue 11, 15 May 2006, Pages 2698–2706
نویسندگان
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