کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2052950 1074245 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Casein kinase Iε associates with and phosphorylates the tight junction protein occludin
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Casein kinase Iε associates with and phosphorylates the tight junction protein occludin
چکیده انگلیسی

Occludin is an integral-membrane protein that contributes to tight junction function. We have identified casein kinase Iε (CKIε) as a binding partner for the C-terminal cytoplasmic domain of occludin by yeast two-hybrid screening. CKIε phosphorylated occludin and co-localised and co-immunoprecipitated with occludin from human endothelial cells. Amino acids 265–318 of occludin were sufficient for CKIε binding and phosphorylation. Deletion of the C-terminal 48 amino acids of occludin increased CKIε binding and phosphorylation, suggesting that this region inhibits CKIε binding. These data identify CKIε as a novel occludin kinase that may be important for the regulation of occludin.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 580, Issue 9, 17 April 2006, Pages 2388–2394
نویسندگان
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