کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2053021 1074251 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Highly efficient protein trans-splicing by a naturally split DnaE intein from Nostoc punctiforme
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Highly efficient protein trans-splicing by a naturally split DnaE intein from Nostoc punctiforme
چکیده انگلیسی

Protein trans-splicing by the naturally split intein of the gene dnaE from Nostoc punctiforme (Npu DnaE) was demonstrated here with non-native exteins in Escherichia coli. Npu DnaE possesses robust trans-splicing activity with an efficiency of >98%, which is superior to that of the DnaE intein from Synechocystis sp. strain PCC6803 (Ssp DnaE). Both the N- and C-terminal parts of the split Npu DnaE intein can be substituted with the corresponding fragment of Ssp DnaE without loss of trans-splicing activity. Protein splicing with the Npu DnaEN is also more tolerant of amino acid substitutions in the C-terminal extein sequence.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 580, Issue 7, 20 March 2006, Pages 1853–1858
نویسندگان
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