کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2053059 1074254 2006 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Energetics of RNA binding by the West Nile virus RNA triphosphatase
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Energetics of RNA binding by the West Nile virus RNA triphosphatase
چکیده انگلیسی

The West Nile virus (WNV) RNA genome harbors the characteristic methylated cap structure present at the 5′ end of eukaryotic mRNAs. In the present study, we report a detailed study of the binding energetics and thermodynamic parameters involved in the interaction between RNA and the WNV RNA triphosphatase, an enzyme involved in the synthesis of the RNA cap structure. Fluorescence spectroscopy assays revealed that the initial interaction between RNA and the enzyme is characterized by a high enthalpy of association and that the minimal RNA binding site of NS3 is 13 nucleotides. In order to provide insight into the relationship between the enzyme structure and RNA binding, we also correlated the effect of RNA binding on protein structure using both circular dichroism and denaturation studies as structural indicators. Our data indicate that the protein undergoes structural modifications upon RNA binding, although the interaction does not significantly modify the stability of the protein.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 580, Issue 3, 6 February 2006, Pages 867–877
نویسندگان
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