کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2053176 | 1074264 | 2005 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Multiple incorporation of non-natural amino acids into a single protein using tRNAs with non-standard structures Multiple incorporation of non-natural amino acids into a single protein using tRNAs with non-standard structures](/preview/png/2053176.png)
The ability to introduce non-natural amino acids into proteins opens up new vistas for the study of protein structure and function. This approach requires suppressor tRNAs that deliver the non-natural amino acid to a ribosome associated with an mRNA containing an expanded codon. The suppressor tRNAs must be absolutely protected from aminoacylation by any of the aminoacyl-tRNA synthetases in the protein synthesizing system, or a natural amino acid will be incorporated instead of the non-natural amino acid. Here, we found that some tRNAs with non-standard structures could work as efficient four-base suppressors fulfilling the above orthogonal conditions. Using these tRNAs, we successfully demonstrated incorporation of three different non-natural amino acids into a single protein.
Journal: FEBS Letters - Volume 579, Issue 30, 19 December 2005, Pages 6769–6774