کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2053258 | 1074268 | 2005 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Lactate utilization in mitochondria prevents Bax cytotoxicity in yeast Kluyveromyces lactis Lactate utilization in mitochondria prevents Bax cytotoxicity in yeast Kluyveromyces lactis](/preview/png/2053258.png)
In a search for the physiological conditions able to suppress the disruption of electron transport through the inner mitochondrial membrane induced by Bax, we found that respiratory substrate – lactate completely abolished Bax toxicity in yeast Kluyveromyces lactis. The effect of lactate was dependent on the presence of cytochrome c, as no effect was observed in the cytochrome c null strain. The investigation of lactate effect on markers of Bax toxicity showed that: (i) oxidation of lactate did not affect the decrease in oxygen consumption, but (ii) lactate was able to diminish the generation of reactive oxygen species and simultaneously to suppress Bax-induced cell death. We show that suppression of Bax lethality in K. lactis can be, in addition to anti-apoptotic proteins, achieved also by the utilization of lactate in the mitochondria.
Journal: FEBS Letters - Volume 579, Issue 23, 26 September 2005, Pages 5152–5156