کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2053269 1074268 2005 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Uptake of cell-penetrating peptides in yeasts
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Uptake of cell-penetrating peptides in yeasts
چکیده انگلیسی

The uptake of different cell-penetrating peptides (CPPs) in two yeast species, Saccharomyces cerevisiae and Candida albicans, was studied using fluorescence HPLC-analyses of cell content. Comparison of the ability of penetratin, pVEC and (KFF)3K to traverse the yeast cell envelope shows that the cellular uptake of the peptides varies widely. Moreover, the intracellular degradation of the CPPs studied varies from complete stability to complete degradation. We show that intracellular degradation into membrane impermeable products can significantly contribute to the fluorescence signal. pVEC displayed highest internalizing capacity, and considering its stability in both yeast species, it is an attractive candidate for further studies.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 579, Issue 23, 26 September 2005, Pages 5217–5222
نویسندگان
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