کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2053354 | 1074273 | 2005 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Gymnemic acids inhibit rabbit glyceraldehyde-3-phosphate dehydrogenase and induce a smearing of its electrophoretic band and dephosphorylation
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کلمات کلیدی
RU, response unitGAPDH, glyceraldehyde-3-phosphate dehydrogenase - GAPDH، glyceraldehyde-3-phosphate dehydrogenaseSPR, surface plasmon resonance - تشدید پلاسمون سطحیRabbit - خرگوشcyclodextrin - سیکلودکسترین ها Dephosphorylation - ضریب نفوذ پذیریglyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Gymnemic acids (GA) inhibited rabbit muscle glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity. Binding of GA to GAPDH was observed by surface plasmon resonance measurement. Incubation of GAPDH with GA induced a smearing of the GAPDH band in SDS–PAGE. The GA-induced smearing was diminished by prior incubation of GA with γ-cyclodextrin or by GA treatment with NAD. GA treatment did not affect the electrophoretic mobility of glucose-6-phosphate isomerase and dehydrogenase. GA treatment diminished the GAPDH band detected by an antibody to phosphoserine, but did not affect the phosphoserine bands of glucose-6-phosphate isomerase and dehydrogenase. These results indicated that GA specifically induced dephosphorylation of GAPDH.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 579, Issue 20, 15 August 2005, Pages 4333–4336
Journal: FEBS Letters - Volume 579, Issue 20, 15 August 2005, Pages 4333–4336
نویسندگان
Yusuke Izutani, Takuya Murai, Toshiaki Imoto, Masatake Ohnishi, Masayuki Oda, Sumio Ishijima,