کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2054455 1543733 2009 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Detection of non-tuberculous mycobacteria in hospital water by culture and molecular methods
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
Detection of non-tuberculous mycobacteria in hospital water by culture and molecular methods
چکیده انگلیسی

Non-tuberculous mycobacteria (NTM) are emerging pathogens in immunocompromised patients. Therefore, it is important for hospitals to consider tap water as a source for these infections. Aim of the study was to establish highly sensitive and specific techniques to detect and identify NTM in hospital drinking water. A Mycobacterium genus-specific 16S rDNA-based real-time LightCycler PCR assay with internal inhibition control and a M. xenopi-specific PCR were developed. Ninety-three water samples from 53 taps from 4 hospitals were investigated. NTM were cultured from 21 of 49 (43%) cold and 32 of 44 (73%) warm water samples. M. chelonae, M. flavescens, M. frederiksbergense, M. gordonae, M. moriokaense, M. mucogenicum, M. vaccae, and M. xenopi were identified with molecular methods. All 93 water samples were positive in the genus-specific PCR. M. xenopi DNA was detected in 40 of 44 (91%) warm and 33 of 49 (67%) cold water samples including 45 of 65 (69%) M. xenopi culture-negative samples. In conclusion, selective culture followed by molecular identification methods enabled detection of rare species of NTM in hospital drinking water and may be used in further studies investigating the epidemiology of NTM in water samples. The real-time PCR assays have a high sensitivity and allow rapid quantification of mycobacterial DNA in water samples.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Medical Microbiology - Volume 299, Issue 4, April 2009, Pages 281–290
نویسندگان
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