کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2054585 1543752 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Intracellular survival and replication of Neisseria meningitidis in human brain microvascular endothelial cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
Intracellular survival and replication of Neisseria meningitidis in human brain microvascular endothelial cells
چکیده انگلیسی

To cause meningitis the extracellular pathogen Neisseria meningitidis has to traverse the blood–cerebrospinal fluid (B–CSF) barrier. Postulating a transcellular passage, meningococci (MC) have been shown to adhere to and enter B–CSF barrier forming human brain microvascular endothelial cells (HBMEC). Furthermore, electron microscopy studies demonstrated that intracellular MC reside within membrane-bound compartments, both solitary and in groups. To investigate the ability of MC to survive and replicate intracellularly, prolonged gentamicin protection assays were performed. Encapsulated bacteria were found to survive and, after an initial delay, to replicate within HBMEC, whereas the number of intracellular capsule-deficient mutants decreased continuously. This strongly suggests that the capsule plays a pivotal role in the intracellular survival of MC. Further investigations were initiated to characterise the membrane-bound compartment, the Neisseria-containing vacuole (NCV). Immunfluorescence microscopy studies showed that NCVs interact with the endocytic pathway acquiring the early endosomal marker protein, transferrin receptor (TfR), and the late endosomal/lysosomal marker protein Lamp-1.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Medical Microbiology - Volume 296, Issue 8, 4 December 2006, Pages 553–558
نویسندگان
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