کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2058724 1543969 2014 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A simple high performance liquid chromatography method for determination of rebamipide in rat urine
ترجمه فارسی عنوان
روش ساده کروماتوگرافی مایع با کارایی بالا برای تعیین ربامایپید در ادرار موش صحرایی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
چکیده انگلیسی

Rebamipide is a mucoprotective agent commonly used to prevent nonsteriodal anti-inflammatory drug-induced gastrointenstinal side effects [1]. Human plasma and urine analysis of rebamipide utilizing high performance liquid chromatography (HPLC) have been reported [2]. Recently, we reported on the plasma levels of rebamipide in presense or absence of celecoxib or diclofenac in rats [3] using a modified HPLC method of detection developed by Jeoung et al. [4]. To tailor the method towards use in urinary rebamipide extraction and analysis, the following modifications were made:
• To compensate for high concentrations of rebamipide found in urine, a new rebamipide stock solution was prepared with a final concentration of 50,000 ng/mL.
• Rat urine calibration standards were obtained within the range of 50–1000 ng/mL and 1000–50,000 ng/mL.
• Plasma samples were replaced with urine samples.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: MethodsX - Volume 1, 2014, Pages 49–55
نویسندگان
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