Description of a novel indole-oxidizing bacterium Pseudomonas indoloxydans sp. nov., isolated from a pesticide-contaminated site

A Gram-negative, deep brown-pigmented Gammaproteobacteria, strain IPL-1T, capable of oxidizing indole was isolated from a lindane-contaminated site and subjected to a polyphasic taxonomic study. Most of the physiological and biochemical properties, major fatty acids (C18:1ω7c, C16:1ω7c/iso C15:0 2OH and C16:0), estimated DNA G+C content (67.2 mol%) and 16S rRNA gene sequence analysis showed that strain IPL-1T belonged to the genus Pseudomonas. Strain IPL-1T exhibited highest 16S rRNA gene sequence similarity with Pseudomonas pseudoalcaligenes (99.0%), followed by Pseudomonas alcaliphila (98.7%), Pseudomonas oleovorans (98.3%), Pseudomonas nitroreducens (98.0%), Pseudomonas mendocina (97.6%) and Pseudomonas stutzeri (97.4%). However, the DNA–DNA relatedness values between strain IPL-1T and the closely related taxa were between 22% and 61%. On the basis of differential phenotypic characteristics and genotypic distinctiveness, strain IPL-1T should be classified within the genus Pseudomonas as a novel species, for which the name Pseudomonas indoloxydans is proposed. The type strain is IPL-1T (=MTCC 8062T=JCM 14246T).