کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2063412 | 1076676 | 2007 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Phylogenetic analysis of enteric species of the family Enterobacteriaceae using the oriC-locus Phylogenetic analysis of enteric species of the family Enterobacteriaceae using the oriC-locus](/preview/png/2063412.png)
Phylogenetic analysis of 38 enteric species belonging to the Enterobacteraceae family was carried out using the non-coding locus oriC, the chromosomal replication origin. The oriC loci were amplified with conserved oligonucleotides and the PCR fragments were sequenced directly. The results establish a phylogenetic tree for the classification of different species of the genera Escherichia, Shigella, Salmonella, Enterobacter, Citrobacter, Klebsiella, Raoultella, Kluyvera, Cedecea and Buttiauxella. Functional important protein-binding sites located in oriC are well conserved throughout the enteric group. More over, due to a high overall divergence value phylogenetic trees were robust and well supported by bootstrap analysis. In comparison with 16S rDNA analysis, the oriC sequences indicated a greater evolutionary divergence for bacteria. We propose that the oriC locus might be a suitable phylogenetic marker for the identification and classification of bacteria, in particular for closely related species.
Journal: Systematic and Applied Microbiology - Volume 30, Issue 3, 19 April 2007, Pages 180–188