کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2063414 1076676 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Arsukibacterium ikkense gen. nov., sp. nov, a novel alkaliphilic, enzyme-producing γ-Proteobacterium isolated from a cold and alkaline environment in Greenland
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک بوم شناسی، تکامل، رفتار و سامانه شناسی
پیش نمایش صفحه اول مقاله
Arsukibacterium ikkense gen. nov., sp. nov, a novel alkaliphilic, enzyme-producing γ-Proteobacterium isolated from a cold and alkaline environment in Greenland
چکیده انگلیسی

A novel aerobic, Gram-negative, non-pigmented bacterium, GCM72T, was isolated from the alkaline, low-saline ikaite columns in the Ikka Fjord, SW Greenland. Strain GCM72T is a motile, non-pigmented, amylase- and protease-producing, oxidase-positive, and catalase-negative bacterium, showing optimal growth at pH 9.2–10.0, at 15 °C, and at 3% (w/v) NaCl. Major fatty acids were C12:0 3-OH (12.2±0.1%), C16:00 (18.0±0.1%), C18:1ω7c (10.7±0.5%), and summed feature 3 comprising C16:1ω7c and/or iso-C15:0 2-OH (36.3±0.7%). Phylogenetic analysis based on 16S rRNA gene sequences showed that isolate GCM72T was most closely related to Rheinheimera baltica and Alishewanella fetalis of the γ-Proteobacteria with a 93% sequence similarity to both. The G+C content of DNA isolated from GCM72T was 49.9 mol% and DNA–DNA hybridization between GCM72 T and R. baltica was 9.5%. Fatty acid analysis and G+C content supports a relationship primarily to R. baltica, but several different features, such as a negative catalase-response and optimal growth at low temperature and high pH, together with the large phylogenetic distance and low DNA similarity to its closest relatives, lead us to propose a new genus, Arsukibacterium, gen. nov., with the new species Arsukibacterium ikkense sp. nov. (type strain is GCM72T).

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Systematic and Applied Microbiology - Volume 30, Issue 3, 19 April 2007, Pages 197–201
نویسندگان
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