کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2063968 1544116 2016 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Description of Loxtox protein family and identification of a new group of Phospholipases D from Loxosceles similis venom gland
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
Description of Loxtox protein family and identification of a new group of Phospholipases D from Loxosceles similis venom gland
چکیده انگلیسی


• Characterization of 23 complete Loxtox proteins and 8 incomplete protein sequences from the Loxosceles similis spider.
• Analysis of the expression pattern of each described Loxtox protein.
• Identification of a new group of Phospholipases D for Loxosceles.

Envenoming resulting from Loxosceles spider bites (loxoscelism) is a recognized public health problem in Brazil. However, the pathophysiology of loxoscelism caused by L. similis bites, which is widespread in Brazil, remains poorly understood. In the present work, the RNA sequencing (RNA-Seq – Next Generation sequencing - NGS) of the L. similis venom gland was performed to identify and analyze the sequences of the key component phospholipase D. The sequences were aligned based on their classical domains, and a phylogenetic tree was constructed. In the bioinformatics analysis, 23 complete sequences of phospholipase D proteins were found and classified as Loxtox proteins, as they contained the characteristic domains of phospholipase D: the active site, the Mg2+-binding domain, and the catalytic loop. Three phospholipase D sequences with non-canonical domains were also found in this work. They were analyzed separately and named PLDs from L. similis (PLD-Ls). This study is the first to characterize phospholipase D sequences from Loxosceles spiders by RNA-Seq. These results contribute new knowledge about the composition of L. similis venom, revealing novel tools that could be used for pharmacological, immunological, and biotechnological applications.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicon - Volume 120, 15 September 2016, Pages 97–106
نویسندگان
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