کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2065594 1076929 2008 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A hyaluronidase from Potamotrygon motoro (freshwater stingrays) venom: Isolation and characterization
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
A hyaluronidase from Potamotrygon motoro (freshwater stingrays) venom: Isolation and characterization
چکیده انگلیسی

Freshwater stingrays (Potamotrygon motoro) are known to cause human accidents through a sting located in its tail. In the State of Goiás, this accident happens especially during the fishing season of the Araguaia River. The P. motoro venom extracted from the sting presented hyaluronidase activity. The enzyme was purified by gel filtration on Sephacryl S-100 and ion-exchange chromatography on SP-Sepharose. A typical procedure provided 376.4-fold purification with a 2.94% yield. The molecular weight of the purified enzyme was 79 kDa as estimated by gel filtration on Sephacryl S-100. The Km and Vmax values for hyaluronidase, using hyaluronic acid as substrate, were 4.91 μg/ml and 2.02 U/min, respectively. The pH optimum for the enzyme was pH 4.2 and maximum activity was obtained at 40 °C. The hyaluronidase from P. motoro was shown to be heat instable, being stabilized by bovine albumin and DTT, and inhibited by Fe2+, Mn2+, Cu2+ and heparin.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicon - Volume 51, Issue 6, May 2008, Pages 1060–1067
نویسندگان
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