کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2066831 1077301 2007 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Isolation and characterization of a novel P-II class snake venom metalloproteinase from Trimeresurus stejnegeri
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
Isolation and characterization of a novel P-II class snake venom metalloproteinase from Trimeresurus stejnegeri
چکیده انگلیسی

Stejnitin, a novel class P-II snake venom metalloproteinase (SVMP) with a molecular weight of about 35 kDa, was purified from Trimeresurus stejnegeri venom. The cDNA of stejnitin encoded a polypeptide of 295 amino acid residues which comprises a signal peptide, proprotein, metalloproteinase domain, spacer and disintegrin domain. The protein sequence deduced from cDNA was confirmed by peptide mass fingerprinting analysis. It is highly homologous to the members of subclass P-IIa SVMPs which comprises metalloproteinase and disintegrin together. Results from DNA fragmentation and flow cytometry analysis also indicated that stejnitin is able to induce apoptosis of ECV304 cells (R=0.908, P=0.012).

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicon - Volume 49, Issue 7, 1 June 2007, Pages 889–898
نویسندگان
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